Heavy metal hypertolerant eukaryotic aldehyde dehydrogenase isolated from metal contaminated soil by metatranscriptomics approach

摘要: Abstract Constant addition of heavy metal pollutants in soil resulting from anthropogenic activities can prove detrimental to both macro and micro life forms inhabiting the ecosystem. The potential functional roles eukaryotic microbes such environment were explored this study by metatranscriptomics approach. Sized cDNA libraries, library A ( 1 kb) constructed RNA screened for copper (Cu) tolerance genes using sensitive yeast mutant strain cup1Δ. Screening libraries yielded different clones capable growing Cu amended medium. In present investigation, one transcripts PLCc38 C was characterized tested its ability tolerate metals mutants. Sequence analysis showed homology with aldehyde dehydrogenase (ALDH) tolerating high concentrations (150–1000 μM). Aldeyde dehydrogenases are stress response enzymes eliminating toxic levels aldehydes generated due abiotic environmental stresses. also provided wide range Cd (40–100 μM), Zn (10–13 mM) Co (2–50 mM) concentrations. Oxidative confirmed presence H2O2. This proves that is a potent gene associated which could be used revegetate polluted or as biomarker detection contamination.