Prussian blue–Au nanocomposites actuated hemin/G-quadruplexes catalysis for amplified detection of DNA, Hg2+ and adenosine triphosphate

作者: Guangfeng Wang , Ling Chen , Yanhong Zhu , Xiuping He , Gang Xu

DOI: 10.1039/C4AN00897A

关键词:

摘要: In this paper, horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) and Prussian blue (PB)–gold (Au) nanocomposites were designed as versatile electrochemical sensing platforms for the amplified detection of DNA, Hg2+ adenosine triphosphate (ATP). By conjugation target probe with capture probe, a conformational change resulted in formation HRP-DNAzyme on PB–Au modified electrode. The redox (red) was efficiently carried out presence H2O2, which PB acted mediator stimulating biocatalytic functions actuated catalytic cycle bringing an signal. Specific recognition ATP allowed selective amperometric molecule. limits 50 nM, 30 pM 3 respectively. highlight work is that between adequately utilized amplification platform sensing. novel electrocatalytic biosensor involving only one-step incubation exhibited wide linear range, low limit, satisfactory selectivity operational stability. proposed approach provided ease-of-use universal reporting system simple design easy operations.

参考文章(42)
Jie Zhao, Youyu Zhang, Haitao Li, Yanqing Wen, Xiaoyu Fan, Fanbo Lin, Liang Tan, Shouzhuo Yao, Ultrasensitive electrochemical aptasensor for thrombin based on the amplification of aptamer-AuNPs-HRP conjugates. Biosensors and Bioelectronics. ,vol. 26, pp. 2297- 2303 ,(2011) , 10.1016/J.BIOS.2010.09.056
Lin He, Michael D. Musick, Sheila R. Nicewarner, Frank G. Salinas, Stephen J. Benkovic, Michael J. Natan, Christine D. Keating, Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization Journal of the American Chemical Society. ,vol. 122, pp. 9071- 9077 ,(2000) , 10.1021/JA001215B
Xiaowu Tang, Sarunya Bansaruntip, Nozomi Nakayama, Erhan Yenilmez, Ying-lan Chang, Qian Wang, None, Carbon nanotube DNA sensor and sensing mechanism. Nano Letters. ,vol. 6, pp. 1632- 1636 ,(2006) , 10.1021/NL060613V
Bernard Munge, Guodong Liu, Greg Collins, Joseph Wang, Multiple Enzyme Layers on Carbon Nanotubes for Electrochemical Detection Down to 80 DNA Copies Analytical Chemistry. ,vol. 77, pp. 4662- 4666 ,(2005) , 10.1021/AC050132G
Yali Yuan, Guangpeng Liu, Ruo Yuan, Yaqin Chai, Xianxue Gan, Lijuan Bai, Dendrimer functionalized reduced graphene oxide as nanocarrier for sensitive pseudobienzyme electrochemicalaptasensor Biosensors and Bioelectronics. ,vol. 42, pp. 474- 480 ,(2013) , 10.1016/J.BIOS.2012.11.003
Maogen Zhang, Audrey Smith, Waldemar Gorski, Carbon Nanotube−Chitosan System for Electrochemical Sensing Based on Dehydrogenase Enzymes Analytical Chemistry. ,vol. 76, pp. 5045- 5050 ,(2004) , 10.1021/AC049519U
Ai-Xian Zheng, Juan Li, Jin-Ru Wang, Xiao-Rong Song, Guo-Nan Chen, Huang-Hao Yang, Enzyme-free signal amplification in the DNAzyme sensor via target-catalyzed hairpin assembly Chemical Communications. ,vol. 48, pp. 3112- 3114 ,(2012) , 10.1039/C2CC30305A
Ute Resch-Genger, Markus Grabolle, Sara Cavaliere-Jaricot, Roland Nitschke, Thomas Nann, Quantum dots versus organic dyes as fluorescent labels Nature Methods. ,vol. 5, pp. 763- 775 ,(2008) , 10.1038/NMETH.1248
Daren J. Caruana, Thierry de Lumley-Woodyear, Adam Heller, Enzyme-Amplified Amperometric Detection of Hybridization and of a Single Base Pair Mutation in an 18 Base Oligonucleotide on a 7 µm Diameter Microelectrode Journal of the American Chemical Society. ,vol. 121, pp. 769- 774 ,(1999) , 10.1007/978-1-4615-1231-8_6
Katie A. Edwards, Antje J. Baeumner, DNA-oligonucleotide encapsulating liposomes as a secondary signal amplification means. Analytical Chemistry. ,vol. 79, pp. 1806- 1815 ,(2007) , 10.1021/AC061471S