SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.
作者： Carmen L Loureiro , Rossana C Jaspe , Pierina D´ Angelo , José L Zambrano , Lieska Rodriguez
DOI: 10.1371/JOURNAL.PONE.0247196
关键词:
摘要: SARS-CoV-2 is the new coronavirus responsible for COVID-19 disease. The first two cases of were detected in Venezuela on March 13, 2020. aim this study was genetic characterization Venezuelan isolates. A total 7 full genome sequences obtained by Sanger sequencing, from patients different regions Venezuela, mainly beginning epidemic. Ten out 11 isolates (6 complete genomes and 4 partial spike genomic regions) belonged to lineage B, bearing D614G mutation Spike protein. Isolates outbreak that occurred Margarita Island harbored an in-frame deletion its sequence, without amino acids 83-85 NSP1 ORF1. search deletions 48,635 showed gene exhibit highest frequency along whole genome. Structural analysis suggests a change N-terminal domain with presence deletion. In contrast, circulating later island lacked deletion, suggesting introductions after outbreak. conclusion, high diversity found predominance mutation. small seemed be associated strain carrying protein, but these do not seem larger which started July.
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