Characterization of Escherichia coli strains producing heat-stable enterotoxin b (STb) isolated from humans with diarrhea.

摘要: Two of 49 cytolethal distending toxin-producing strains Escherichia coli isolated from human stools contained the gene coding for heat-stable enterotoxin b (STb), as detected by a colony hybridization assay. The STb was found to be on 70-kb plasmid also heat-labile (pLT-I). Restriction endonuclease analysis showed isolates similar in porcine strains. Moreover, enzymatic amplification based oligonucleotide primers designed sequence, expected portion amplified E. these bioactive rat jejunal loops and with an enzyme-linked immunosorbent assay using polyclonal antiserum raised against purified toxin. Images