Stability of fluorescein labeled dextrans in vivo and in vitro.

摘要: Abstract Fluorescein thiocarbamoyl dextrans (FITC-dextrans) were studied with respect to their potential use as tracer substances in circulatory research. It was found that the preparations did not contain any detectable free fluorescein. FITC-dextrans could conveniently be determined by fluorometry concentration range of 0.05–30 μg/ml. Gel filtration experiments demonstrated fluorescein label evenly distributed molecular weight dextran molecules, and carbamoyl-dextran linkage perfectly stable both vitro vivo conditions. Intravenous infusion FITC-dextran into rabbits resulted plasma disappearance curves similar those obtained corresponding unlabeled preparations. Consequently, should prove valuable tracers for study many aspects circulation.