First isolation of an antifungal lipid transfer peptide from seeds of a Brassica species

摘要: An antifungal peptide with a molecular mass of 9412 and an N-terminal sequence exhibiting notable homology to those lipid transfer proteins was isolated from seeds the vegetable Brassica campestris. The purification protocol entailed ion exchange chromatography on Q-Sepharose, affinity Affi-gel blue gel, by fast protein liquid (FPLC) Mono S, gel filtration FPLC Superdex column. adsorbed S. It inhibited mycelial growth in Fusarium oxysporum Mycosphaerella arachidicola IC(50) value 8.3 microM 4.5 microM, respectively. exhibited dose-dependent binding lyso-alpha-lauroyl phosphatidylcholine. present findings constitute first report non-specific species.