Gene expression patterns in human prostate stem cell differentiation

摘要: The identification of phenotypic differences between stem cells (SCs) and their more differentiated counterparts is crucial for designing novel SC-based therapeutics prostate cancer (CaP). RARRES1 LXN were identified as two homologous genes whose expression was highly significantly down-regulated in the SC fraction compared to epithelial cells. overall aim this study investigate expression, regulation function SC-silenced LXN, potential interacting partner CPA4 differentiation CaP. We showed that genes, induced by pro-differentiation agent all-trans retinoic acid (atRA). AtRA a higher extent most than fraction, suggesting sub-population less responsive atRA. Importantly, siRNA suppression enhanced properties primary cultures, shown significant increase colony forming ability. Expression both co-ordinately repressed DNA methylation CaP cell lines inhibition increased invasive capacity which also fully rescued an inhibitory effect Despite homology adjacent location on chromosome 3, we provide evidence reside within different sub-cellular compartments; not plasma membrane protein previously supposed but located endoplasmic reticulum, while localised nucleus cells. These data results identifying tumour suppressor regulated, suppress invasion ability Work now should be focussed determining whether re-administration would valid strategy treatment, potentially eradication, CaP.