Metabolomics Studies To Decipher Stress Responses in Mycobacterium smegmatis Point to a Putative Pathway of Methylated Amine Biosynthesis.

摘要: ABSTRACT Mycobacterium smegmatis, the saprophytic soil mycobacterium, is routinely used as a surrogate system to study human pathogen tuberculosis. It has also been reported an opportunistic in immunocompromised hosts. In addition, it can exist several ecological setups, thereby suggesting its capacity adapt variety of environmental cues. this study, we employed untargeted proton nuclear magnetic resonance (1H-NMR)-based metabolomics identify metabolites and metabolic pathways critical for early adaptive responses acidic stress, oxidative nutrient starvation smegmatis. We identified 31, 20, 46 that showed significant changes levels response acidic, oxidative, stresses, respectively. Pathway analyses perturbations purine-pyrimidine, amino-acid, nicotinate-nicotinamide, energy metabolism pathways. Besides these, differential intermediary involved α-glucan biosynthesis pathway were observed. detected high organic osmolytes, methylamine, betaine during stress. Further, tracing these osmolytes through computational search tools, gene expression studies (using reverse transcription-PCR [RT-PCR]), enzyme assays, presence putative betaine, dimethylamine previously unreported IMPORTANCE Alterations metabolite provide fast direct means regulate enzymatic reactions and, therefore, This documents, first time, occur smegmatis three namely, starvation. These stresses are faced by intracellular mycobacteria infection therefore may be extended frame therapeutic interventions pathogenic mycobacteria. addition amino acid, found affected different novel methylamine was present