Calibration of gel permeation chromatography materials for use with humic acid
作者： R. S. CAMERON , R. S. SWIFT , B. K. THORNTON , A. M. POSNER
DOI: 10.1111/J.1365-2389.1972.TB01666.X
关键词:
摘要: Summary Three common gel-chromatography materials have been calibrated for humic acid, using fractionated samples the molecular weights of which were calculated by combining sedimentation coefficients determined on ultracentrifuge with diffusion coefficients. The gel found to following fractionation ranges acid when tris buffer was used: Sephadex G-100, 2500 100000; Bio Gel P-150, below 1000 40000; Sepharose 6B, 1500 2500000. Borax appeared extend range G-100 lower weights. A brief comparison is made retention behaviours proteins, and dextrans materials.
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