Transcriptome differences in adipose stromal cells derived from pre- and postmenopausal women

摘要: As the population ages, an increasing number of postmenopausal women are donors adipose stromal cells (ASCs) and may benefit from autologous ASC-related treatments. However, effect menopausal status on ASCs has not been investigated. RNA sequencing data were downloaded, differentially expressed genes (DEGs) identified. Hierarchical clustering, Gene Ontology, pathway analyses applied to DEGs. Two gene coexpression network analysis approaches DEGs provide a holistic view preserve interactions. Hub identified, their expression profiles examined with clinical samples. pre- co-cultured monocytes T determine immunoregulatory role. In total, 2299 identified presented distinct between women. Ontology revealed some fertility-, sex hormone-, immune-, aging-, angiogenesis-related terms pathways. networks constructed, top hub genes, including TIE1, ANGPT2, RNASE1, PLVAP, CA2, MPZL2, consistent two approaches. Expression samples consistent. elicit M1 polarization, while counterparts facilitate CD3/4+ cell proliferation. The present study reveals transcriptome differences in derived provides views by preserving interactions via analysis. this could serve as potential targets enhance therapeutic ASCs.