Quantitative and wide-ranging profiling of phospholipids in human plasma by two-dimensional liquid chromatography/mass spectrometry.

摘要: Normal-phase or reverse-phase liquid chromatography has been used in phospholipidomics for lipid separation prior to mass spectrometry analysis. However, using a single mode is often inadequate, as high-abundance phospholipids can mask large numbers of low-abundance lipids interest. In order detect and quantify phospholipids, we present novel two-dimensional (2D) approach sensitive quantitative global analysis phospholipids. The methodology monitors individual glycerolipids through the use new normal-phase, solid-phase extraction procedure, followed by molecular characterization relative quantification an ion-trap Orbitrap equipped with chromatograph, data processing MS++ software. CV (%) peak area each standard was less than 15% this method. When method applied liver sample, could more phosphatidylserine (PS) compare...