Quantitative analysis of benzyl isothiocyanate in Salvadora persica extract and dental care herbal formulations using reversed phase C18 high-performance liquid chromatography method

摘要: Context: Benzyl isothiocyanate is the active antimicrobial agent in Salvadora persica (siwak) widely used Islamic countries for oral hygiene. Aims: Quantification of benzyl ethanol extract S. and some dental care herbal formulations labeled to contain siwak. Settings Design: Simple sensitive high-performance liquid chromatography method was designed. Subjects Methods: Separation achieved on reverse phase C18 (250 mm × 4.6 mm, 5 μ ) column with a mobile comprising acetonitrile water (1:1). The detection carried out at 190 nm using ultra violet-visible detector. flow rate kept 1 mL/min. Results: A sharp well-defined peak obtained retention time 9.322 ± 0.3 min. Linear regression analysis data calibration plot showed good linear relationship between response concentration range 0.5–500 μg/mL coefficient (r2) 0.9977. validated accuracy, precision, robustness, sensitivity. All parameters examined met current recommendations International Conference Harmonization guidelines validation. Conclusions: applied quantification siwak extract, powder, mouth wash, toothpaste claimed developed found specific, simple, selective, reliable routine use quality control different commercially available products. Abbreviations used: RP18: Reversed C18, HPLC: High performance chromatography, UV: Ultra violet, r2: coefficient, ICH: international conference harmonization, TLC: Thin layer CHCl3: Chloroform, v/v: volume/volume, RSD: Relative standard deviation, LOD: Limit detection, LOQ: quantification.