KCNJ1 inhibits tumor proliferation and metastasis and is a prognostic factor in clear cell renal cell carcinoma

摘要: Potassium inwardly rectifying channel, subfamily J, member 1 (KCNJ1), as an ATP-dependent potassium plays essential role in balance. KCNJ1 variation is associated with multiple diseases, such antenatal Bartter syndrome and diabetes. However, the of clear cell renal carcinoma (ccRCC) still unknown. Here, we studied expression function ccRCC. The was evaluated ccRCC tissues lines by quantitative real-time PCR (qRT-PCR), Western blot, immunohistochemistry analysis. relationship between clinicopathological characteristics analyzed. p3xFLAG-CMV-14 vector containing constructed used for transfecting 786-O Caki-2. effects on proliferation, invasion, apoptosis were detected using proliferation assay, transwell flow cytometry, respectively. We found that low-expressed samples lines, its level significantly tumor pathology grade (P = 0.002) clinical stage 0.023). Furthermore, a prognostic factor patient’s survival 0.033). re-expression Caki-2 inhibited cancer growth invasion promoted apoptosis. Moreover, knockdown HK-2 cells proliferation. Collectively, these data highlight KCNJ1, poor prognosis, important metastasis.