Deoxyribonuclease I in mammalian tissues. Specificity of inhibition by actin.

摘要: Enzymes of the DNase I class, similar to bovine pancreatic with respect molecular weight and ionic pH requirements, were found in various tissues rat. Their analysis was facilitated by a method for detection nucleases crude extracts after polyacrylamide gel electrophoresis presence sodium dodecyl sulfate subsequent renaturation enzymes. High levels digestive tissues, such as parotid submaxillary salivary glands lining small intestine., Appreciable present lymph node, kidney, heart, prostate gland, seminal vesicle. No activity extracts. However, under some conditions, rich proteases gave poor recovery I. Fourteen other showed little or no Inhibition enzymes rabbit muscle actin examined both gels solution. Actin inhibited well enzyme, but did not inhibit any This species specificity inhibition makes it unlikely that very strong association between monomeric is general significance cellular function.