Regulatory Role of Arginine-Specific Mono(ADP-Ribosyl)Transferase in Muscle Cells

摘要: Earlier we demonstrated that meta-iodobenzylguanidine (MIBG), a specific inhibitor of arginine mono-ADP-ribosylation blocks proliferation and differentiation chick skeletal myogenic cells in culture (Exp. Cell Res., 1992, 201: 33-42). Membrane fractions from 4-day, myotube cultures embryonic muscle were incubated with 32P-NAD+. Several proteins labeled, but labeling two bands about 53 36 kDa appeared to be due arginyl ADP-ribosylation. Immunoprecipitation D3 monoclonal antibody the intermediate filament protein desmin, SDS-PAGE autoradiography band contained this desmin is ADP-ribosylated by endogenous arginine-specific mono(ADP-ribosyl)transferase Res.,1996, press). Desmin muscle-specific protein, it appears one first expressed during terminal differentiation. We have examined whether can use polyclonal antibodies for residues. found soluble present 5-6 day cell contains ADP-ribose, demonstrating cells. also purified avian antigenic material reacts these antibodies. In both cases, NaCl had no effect on reactivity, NH2OH did, which consistent an arginine-ADPR linkage. summary, results suggest ADP-ribosylation important regulatory mechanism differentiating cells, substrate modification