Lubrication and wear properties of grafted polyelectrolytes, hyaluronan and hylan, measured in the surface forces apparatus.

摘要: Hyaluronan is believed to have an important function in the boundary biolubrication of articular cartilage. Using a Surface Forces Apparatus, we tested tribological properties surface bound, rather than "free" hyaluronan. The grafting process polyelectrolyte included either biological route via HA-binding protein or chemical reaction covalently bind polymer lipid bilayer coated surface. In another reaction, constructed with grafted hylan (crosslinked hyaluronan). We studied normal and shear forces between these surfaces. None systems demonstrated comparable lubrication that found cartilage surfaces except at very low loads. Both hyaluronan generated coefficients friction 0.15 0.3. Thus, polysaccharide, which constituent lamina splendens (outermost layer), not expected be responsible molecule for great lubricity cartilage; however, it may contribute load bearing wear protection This was concluded from results hylan, where thin gel layer sufficient shield underlying damage even applied pressures over 200 atmospheres during shear. Our study shows coefficient requirement for, necessarily measure of, protection.