Comparison of anaerobic digestion strategies of nitrogen-rich substrates: Performance of anaerobic reactors and microbial community diversity.

摘要: Abstract In the present study, application of different operating strategies on performance three continuous stirred tank reactors digesting chicken manure at mesophilic temperature and constant organic loading rate (OLR) 3.5 gVS L−1 d−1 was investigated. Control reactor (RC) (RH) with decreasing hydraulic retention time (HRT) had comparable specific biogas production (SBP) maximum values 334–351 mL g−1VS (added) during days 84–93, while SBP from zeolites (RZ) higher achieved 426–432 mL g−1VS (added). Attachments microorganisms to zeolite particles as operational environment, exchanged cations released well lower total ammonium nitrogen (TAN) levels observed in RZ (6.2–6.3 g L−1; 71–93) compared RC (6.6–6.9 g L−1; resulted a more effective process RZ. Moreover, microbial community structure dynamics were comprehensively characterized using Illumina sequencing, pyrosequencing T-RFLP analysis 16S rRNA genes. Methanogenic archaeal activity additionally assessed by expressed mcrA genes encoding alpha subunit methyl-CoM reductase. Within major class Clostridia, Caldicoprobacter, Alkaliphilus, Gallicola, Sporanaerobacter Tepidimicrobium spp. notable bacteria developed operation all tested reactors. Archaeal communities dominated methanogens belonging genus Methanosarcina followed Methanoculleus experimental period. Results this study indicate that attachment environment might have led high ammonia concentrations.