Effect of Artificially Inserted Intron on Gene Expression in Saccharomyces cerevisiae
作者： TADANORI YOSHIMATSU , FUMIKIYO NAGAWA
关键词:
摘要: ABSTRACT The intron of the yeast RP51A gene was cloned with precision using polymerase chain reaction (PCR) amplification method, and then inserted into several different positions URA3 as well PGK–lacZ fusion without introduction additional exon sequences. Analysis transcripts these genes showed that an near transcription start site spliced out efficiently, whereas same sequences 200 bp or further downstream were not, resulting in a reduced level mRNA. These results explain why intron-containing usually have 5′ end.
liebertonline.com参考文章(23)
A.M. Miller, The yeast MATa1 gene contains two introns. The EMBO Journal. ,vol. 3, pp. 1061- 1065 ,(1984) , 10.1002/J.1460-2075.1984.TB01927.X
A. Yoshimoto, K. Umezu, K. Kobayashi, K. Tomita, [11] Orotidylate decarboxylase (yeast) Methods in Enzymology. ,vol. 51, pp. 74- 79 ,(1978) , 10.1016/S0076-6879(78)51013-6
M J Casadaban, J Chou, S N Cohen, In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. Journal of Bacteriology. ,vol. 143, pp. 971- 980 ,(1980) , 10.1128/JB.143.2.971-980.1980
John L. Woolford, Nuclear pre-mRNA splicing in yeast. Yeast. ,vol. 5, pp. 439- 457 ,(1989) , 10.1002/YEA.320050604
Claudio W. Pikielny, John L. Teem, Michael Rosbash, Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing Cell. ,vol. 34, pp. 395- 403 ,(1983) , 10.1016/0092-8674(83)90373-2
John L. Teem, Nadja Abovich, Norbert F. Kaufer, Willam F. Schwindinger, Jonathan R. Warner, Allison Levy, John Woolford, R.J. Leer, M.M.C. Van Raamsdonk-Duin, W.H Mager, R.J. Planta, L. Schultz, J.D. Friesen, Howard Fried, Michael Rosbash, A comparison of yeast ribosomal protein gene DNA sequences Nucleic Acids Research. ,vol. 12, pp. 8295- 8312 ,(1984) , 10.1093/NAR/12.22.8295
J. L. Teem, M. Rosbash, Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 4403- 4407 ,(1983) , 10.1073/PNAS.80.14.4403
R. Saiki, D. Gelfand, S Stoffel, S. Scharf, R Higuchi, G. Horn, K. Mullis, H. Erlich, Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Science. ,vol. 239, pp. 487- 491 ,(1988) , 10.1126/SCIENCE.2448875
Gerald R Fink, Pseudogenes in yeast? Cell. ,vol. 49, pp. 5- 6 ,(1987) , 10.1016/0092-8674(87)90746-X
Mark Rose, Paula Grisafi, David Botstein, Structure and function of the yeast URA3 gene: expression in Escherichia coli. Gene. ,vol. 29, pp. 113- 124 ,(1984) , 10.1016/0378-1119(84)90172-0