Cfp1 is required for gene expression dependent H3K4me3 and H3K9 acetylation in embryonic stem cells

摘要: Background Trimethylation of histone H3 lysine 4 (H3K4me3) accumulates at promoters in a gene activity dependent manner. The Set1 complex is responsible for most H3K4me3 somatic cells and contains the conserved subunit Cfp1, which implicated targeting to CpG islands mammals. In mouse embryonic stem cells, Cfp1 necessary accumulation constitutively active promoters, but not required maintain steady-state transcription associated gene. Results Here we show that instrumental upon rapid transcriptional induction response external stimuli. Surprisingly, ensure appropriate output rather plays specific roles. We also Cfp1-dependent deposition contributes H3K9 acetylation genome wide, suggesting regulates overall dynamics acetyl transferase recruitment. Finally, observe increased antisense start end genes require accurate H3K9ac. Conclusions Our results assign key role establishing promoter chromatin state shed light on how signaling pathways provide context-dependent outcomes.