Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes.

摘要: Background: Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as link between obesity insulin resistance. The aim of this study was develop sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin evaluate serum concentrations normal subjects patients with type 2 diabetes. Methods: Using ELISA developed by two polyclonal antibodies, were measured 90 diabetes compared 74 healthy control subjects. Results: This high specificity sensitivity over the concentration range 0.5–100 ng/ml good percentage recovery (97.1±4.7%) reproducibility (within-day assay, CV=4.8–8.6%; between-day CV=5.6–9.7%). mean sera from diabetic significantly higher than that (mean±S.E.: 20.8±0.7 vs. 14.9±0.5 ng/ml, p<0.001). A moderate positive correlation observed levels body mass indices both (r=0.412, p<0.0003) (r=0.395, p<0.0001). Conclusions: Our will be useful confirm physiological pathophysiological role humans.