Balancing the effect of corona on therapeutic efficacy and macrophage uptake of lipid nanocapsules.

摘要: Several studies have shown the potential of biocompatible lipid nanocapsules as hydrophobic drug delivery systems. Understanding factors that determine interactions these oil-in-water nanoemulsions with cells is a necessary step to guide design most effective formulations. The aim this study was probe ability two surfactants markedly different nature, non-ionic poloxamer, and charged phospholipid, prepare formulations shells composition surface properties. Thus we determined their effects on interaction biological environments. In particular, investigated how shell formulation affected adsorption biomolecules from surrounding fluids nanocapsule (corona formation). A complete physicochemical characterization including an isothermal titration calorimetry (ITC) revealed use poloxamer led marked reduction in number protein-binding sites. Surface hydrophilicity changes corona formation strongly correlated uptake by cancer macrophages. Our results indicate nature concentration can be easily manipulated effectively modulate architecture controlling environmental interactions, thus optimizing functionality for in vivo applications. addition reduce protein binding nanoparticle clearance immune system, but also screens desired cells, leading lower uptake, therapeutic efficacy. need balanced order obtain successful