Genome-wide association mapping for grain yield components and root traits in Brassica juncea (L.) Czern & Coss.

摘要: We used a Diversity Fixed Foundation Set comprising 48 inbred lines of Brassica juncea and representing all the adaption zones crop for association mapping. Extensive phenotypic variations were observed grain yield components root traits under both irrigated restricted moisture conditions. The genotypes differed in their responses to stress. Trait averages declined numerically conditions when compared standard irrigation schedule. Canonical analysis demonstrated importance primary branches seed size as significance drought susceptibility index. Microsatellite markers (158), 18 chromosomes, assess population structure, linkage disequilibrium (LD) panel marker-trait associ- ations (MTA's). A comparison four models (general linear model/GLM(Q-matrix/Q), mixed model: MLM(Q?kinship matrix/K), GLM (principal components/PC) MLM(PC ? K)) showed that GLM(PC) K), incorpo- rating principal kinship matrix, best models. Maximum proportions significant results K). MLM was preferred there fewer false positives than GLM. Thirteen associations detected between molecular agronomic traits. Of these, seven identified normal conditions, six Marker-trait included associated with yield, three size, two secondary one marker each plant height, diameter length. single SB1822-1, repeatedly localized at 17.5 cM (centiMorgans) on chromosome B3. Marker SB3872-3 revealed signif- icant effect (R 2 % = 15.16) 60.9 B5 during first year. Among favorable alleles, SB1822-1 had average positive yield. cnu316-3 maximum effects