Molecular genotyping of methicillin-resistant Staphylococcus aureus via fluorophore-enhanced repetitive-sequence PCR.

摘要: Methicillin resistance in Staphylococcus aureus is a frequent cause of nosocomial and community-acquired infections. Accurate, rapid epidemiologic typing crucial to the identification source spread infectious disease could provide detailed information on generation methicillin-resistant S. (MRSA) strains. The high degree genetic relatedness MRSA strains has precluded use more conventional methods fingerprinting. A DNA fingerprinting method that exploits PCR amplification from repeat sequence described. random chromosomal distribution this provides an ideal target for detecting fragment patterns specific individual Two which oligonucleotide primer based repetitive found Mycoplasma pneumoniae are presented. element sequence-based (rep-PCR) fluorophore-enhanced rep-PCR (FERP) can identify epidemic among background MRSA. combination primers labeled with different fluorescent dyes allowed simultaneous FERP mecA gene detection. Eight fingerprint were observed collected sources. These techniques discriminatory means molecular involved