Genome-wide analysis of membrane transport using yeast knockout arrays.

摘要: Abstract The transport of membrane-bound proteins through post-Golgi compartments depends on the coordinated function multiple genes that direct recognition and routing protein cargoes to their final cellular destination. As many these sorting components are nonessential for viability, genome-wide screening yeast gene-deletion mutant collection provides a useful strategy identification. potential this approach is limited only by availability assays suitable high-throughput colony arrays. Two large-scale phenotypic screens identify novel described here. fluorescence-based Calcofluor white assay identifies mutants with altered plasma membrane localization chitin synthase Chs3, which recycles between cell surface, endosomes, late Golgi. carboxypeptidase Y (CPY) allows distinct Golgi-to-vacuole pathway be identified, due missorting secretion vacuolar hydrolase CPY from cell.