Cell Mimicking Microparticles Influence the Organization, Growth, and Mechanophenotype of Stem Cell Spheroids.

摘要: Substrate stiffness is known to alter cell behavior and drive stem differentiation, though most research in this area has been restricted traditional, two-dimensional culture systems rather than more physiologically relevant, three-dimensional (3D) platforms. In study, we utilized polymer-based, mimicking microparticles (CMMPs) deliver distinct, stable mechanical cues human adipose derived cells 3D spheroid examine changes adipogenic differentiation response mechanophenotype. After 21 days of induction, spheroids containing CMMPs (composite spheroids) stiffened accordance with CMMP elasticity such that the stiffest, ~ 10 kPa, were over 27% stiffer those incorporating compliant, ~ 0.25 kPa CMMPs. Adipogenically induced, cell-only 180% larger 50% compliant matched controls. Interestingly, composite cultured without chemical induction factors dissociated when presented ~ 1 kPa, while mitigated behavior. Gene expression for PPARG FABP4 upregulated 45-fold adipogenically induced samples compared controls but unaffected by elasticity, attributed insufficient cell-CMMP contacts throughout spheroid. summary, mechanically tuned influenced whole-spheroid mechanophenotype stability minimally affected response.