Application of qPCR assays based on haloacids transporter gene dehp2 for discrimination of Burkholderia and Paraburkholderia

摘要: A major facilitator superfamily transporter Dehp2 was recently shown to be playing an important role in transport and biodegradation of haloacids Paraburkholderia caribensis MBA4, is phylogenetically conserved Burkholderia sensu lato. We designed both stricto-specific Paraburkholderia-specific qPCR assays based on dehp2 16S rRNA, validated the 12 bacterial strains. The could detect single species stricto or with high sensitivity discriminate them mixtures specificity over a wide dynamic range relative concentrations. At relatively lower cost compared sequencing-based approach, will facilitate discrimination large number samples. For first time, we report utilization gene for discriminative purpose This enables not only quick decision proper handling putative pathogenic samples group but also future exploitation relevant purposes.