Chapter 4 Molecular Size and Shape in Electrophoresis

摘要: Publisher Summary The molecular charge has no influence on net migration in electrophoresis, if the pore and diameters are identical. In order to decrease scatter of data, it is necessary find a suitable means eliminating differences among different separated species. If an electrophoretic separation carried out gel that size small enough restrict mobility, relationship between distance travelled by particular zone its weight nearly linear. Proteins with higher weights more retarded matrix compared those lower weights. Good results can be obtained within average values weight. It was noticed at beginning development sodium dodecyl sulphate (SDS) procedure addition SDS proteins under conditions which denaturation does not occur, native conformation protein remains preserved even after detergent been added. optical rotatory dispersion (ORD) spectra revealed preservation considerable amount structures, such as a-helix SDS.