Editing the Trypanosoma cruzi genome with zinc finger nucleases
作者： Gabriela Assis Burle-Caldas , Viviane Grazielle-Silva , Melissa Soares-Simões , Gabriela Schumann Burkard , Isabel Roditi
DOI: 10.1016/J.MOLBIOPARA.2017.01.002
关键词:
摘要: Gene function studies in Trypanosoma cruzi, the protozoan parasite that causes Chagas disease, have been hindered by lack of efficient genetic manipulation protocols. In most organisms, insertion and deletion DNA fragments genome are dependent on generation double-stranded break (DSB) repair. By inducing a site-specific DSB, zinc finger nucleases (ZFNs) proven to be useful enhance gene editing many cell types. Using pair ZFNs targeted T. cruzi gp72 gene, we were able generate knockout parasites with improved efficiency compared conventional protocol. We also provide evidence that, repair DSBs generated occurs primarily homologous recombination pathway.
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