MicroRNA signatures associated with immortalization of EBV-transformed lymphoblastoid cell lines and their clinical traits.

摘要: Objective:  MicroRNAs (miRNAs) are negative regulators of gene expression that play important roles in cell processes such as proliferation, development and differentiation. Recently, it has been reported miRNAs related to carcinogenesis. The aim this study was identify associated with terminal immortalization Epstein–Barr virus (EBV)-transformed lymphoblastoid line (LCL) clinical traits. Material Methods:  Hence, we performed miRNA microarray approach early- (p6) late-passage (p161) LCLs. Results Conclusion:  Microarray data showed nine (miR-20b*, miR-28-5p, miR-99a, miR-125b, miR-151-3p, miR-151:9.1, miR-216a, miR-223* miR-1296) were differentially expressed most LCLs during long-term culture. In particular, miR-125b up-regulated all the tested LCLs. miR-216a miR-1296 putative RASGRP3, GPR160, PRKCH XAF1, respectively, which found be culture a previous study. Linear regression analysis miR-200a miR-296-3p correlated triglyceride HbA1C levels, suggesting signatures could provide information on donor’s health. conclusion, our suggests changes specific may required for Thus, would potential marker completion EBV-mediated tumorigenesis.