Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis

摘要: BACKGROUND AND AIM OF THE STUDY Pulmonary sarcoidosis is a chronic granulomatous disease characterized by macrophage and CD4+ T-cell accumulation at the site of inflammation. Analysis cytokine network has substantially improved knowledge on immunopathogenesis sarcoidosis. We hypothesize that neurotrophins (NTs), nerve growth factor (NGF), brain derived neurotrophic (BDNF) NT-3, besides their importance in immune system activities, participate inflammatory disorders repair processes. METHODS The expression NTs NT receptors was assessed broncho alveolar lavage (BAL) macrophages, CD4 CD8 T-cells, from 10 patients with pulmonary sarcoidosis, using molecular biology, Western blotting immunocytochemistry. RESULTS Increased levels high affinity receptor (Trks) transcripts proteins BAL CD8+ T-cells were demonstrated comparison healthy controls. Contrarily to controls, NGF increased macrophages as well BDNF T-cells. An TrkA, TrkB TrkC also noticeable. Furthermore, NT-3 three different cell populations investigated induced during A significant correlation observed between CD4:CD8 ratio, lymphocytosis, radiological stage expression. CONCLUSIONS These findings suggest are exaggeratedly expressed cells may progression modulating functions.