COMPARATIVE STUDIES OF THE EFFECT OF BSA VS FCS AS A SUPPLEMENT IN TCM-199 ON THE IN VITRO MATURATION RATE OF BUFFALO OOCYTES COLLECTED FROM SLAUGHTERHOUSE OVARIES

作者: Yosef Deneke , Prem Singh Yadav , Trilok Nanda , Rajib Deb

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摘要: Availability of developmentally competent buffalo oocytes is critical for in vitro embryo production and application related biotechniques. The objective the present study was to assess effect BSA place FCS as maturation medium supplement on oocytes. Oocytes were aspirated from abattoir ovarian follicles 2-8 mm diameter followed by TCM-199 supplemented with hCG, PMSG containing either 0.4% (group-I) or 10% (groupII). Based cumulus expansion rate, it assessed that among two groups, Group 2 showed signifi cantly higher percentage values (89.1±3.5%) compared 1 (73.9±4.2%).

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