High-Throughput SNP Genotyping to Accelerate Crop Improvement

摘要: Recent advances in next-generation sequencing (NGS) and single nucleotide polymorphism (SNP) genotyping promise to greatly accelerate crop improvement if properly deployed. High-throughput SNP offers a number of advantages over previous marker systems, including an abundance markers, rapid processing large populations, variety systems meet different needs, straightforward allele calling database storage due the bi-allelic nature markers. NGS technologies have enabled whole genome sequencing, providing extensive discovery pools select informative markers for sets germplasm. Highly multiplexed fixed array platforms powerful approaches such as genome-wide association studies. On other hand, routine deployment trait-specific requires flexible, low-cost smaller numbers SNPs across breeding using Fluidigm's Dynamic Arrays™, Douglas Scientific's Array Tape™, LGC's automated running KASP™ At same time, by (GBS) is rapidly becoming popular high-density scans through sequencing. This review will discuss range options available modern breeders integrating into their programs, whether outsourcing service providers or setting up in-house facilities, provide example rice research demonstrated Genotyping Services Lab at International Rice Research Institute.