Studies on the Mechanisms of Hypersensitivity Phenomena XII. An in Vitro Study of the Reaction between Ragweed Pollen Antigen, Allergic Human Serum and Ragweed-Sensitive Human Leukocytes

摘要: Summary The serum from allergic individuals inhibits the antigenically induced release of histamine sensitive human leukocytes. This is in contrast to effect normal serum, which enhances release. On basis this observation, an vitro method for study reaction between ragweed antigen and anti-ragweed activity contained sera has been presented. Over a wide range concentrations, binds constant percentage rather than quantity added antigen. outcome demonstrated be predictable mass action law under limiting condition extreme antibody excess. Kinetic multiplicity studies are compatible with formulation. Determinations antigen-neutralizing capacity provide index those immunoglobulins possess anti-anaphylactic properties. results these determinations differ only slightly when performed on cells different donors. In agreement observation demonstration that concentration cell-bound considerably less does not compete latter Antigen-neutralizing every tested, irrespective previous desensitization therapy. level group untreated its increase as result immunization