An Epichloë festucae homologue of MOB3, a component of the STRIPAK complex, is required for the establishment of a mutualistic symbiotic interaction with Lolium perenne

摘要: Summary In both Sordaria macrospora and Neurospora crassa, components of the conserved STRIPAK (striatin-interacting phosphatase kinase) complex regulate cell–cell fusion, hyphal network development fruiting body formation. Interestingly, a number Epichloe festucae genes that are required for such as noxA, noxR, proA, mpkA mkkA, also establishment mutualistic symbiotic interaction with Lolium perenne. To determine whether MobC, homologue component MOB3 in S. N. is E. fusion symbiosis, mobC deletion strain was generated. The ΔmobC mutant showed reduced rates formed intrahyphal hyphae exhibited enhanced conidiation. Plants infected were severely stunted. Hyphae proliferative pattern growth within leaves perenne increased colonization intercellular spaces vascular bundles. Although still able to form expressoria, structures allowing leaf surface, frequency formation significantly reduced. Collectively, these results show MobC association between L. perenne, plays an accessory role regulation expressorium festucae.