Variables associated with mitochondrial copy number in human blastocysts: what can we learn from trophectoderm biopsies?

摘要: Objective To study the potential variables that affect mitochondrial DNA (mtDNA) content of trophectoderm (TE) cells in blastocysts have undergone TE biopsy. Design Observational retrospective single-center analysis. Setting University-affiliated private in vitro fertilization center. Patient(s) A total 465 consecutive preimplantation genetic screening (PGS) cycles 402 women undergoing testing. Intervention(s) Trophectoderm biopsy performed on testing-aneuploidy (PGT-A). Main Outcome Measure(s) The mtDNA cells. Result(s) We checked possible influence patient characteristics, ovarian stimulation variables, embryo morphology, and culture conditions values. Of all analyzed some such as body mass index (BMI), serum progesterone (P4), aneuploidy, quality had an effect blastocysts. Body a small but positive copy number; BMI values increased, probability producing with above median increased by 6%. For P4 concentration, increase lowered having 39%. Embryo-associated aneuploidy status appeared to number. aneuploid blastocysts, being 42%. Finally, poor more chances carrying higher Conclusion(s) Summarizing, larger quantities are associated condition low TE, well from high Understanding biological meaning human what factors may interfere their is fundamental. Other key gaps, whether correlation exists between biogenesis TE cells, this can be extended inner cell mass, need further addressed. These questions currently investigated.