Regulation of copy number and stability of phage λ derived pTCλ1 plasmid in the light of the dimer/multimer catastrophe hypothesis

摘要: The dimer catastrophe hypothesis has been proposed previously to explain instability of multicopy plasmids whose partitioning is random, contrary low copy number which are stably maintained and actively partitioned. Until now, this investigated using ColE1 plasmids. However, for more detailed testing the dimer/multimer hypothesis, one should use a plasmid can be at either or high still possesses same mechanism replication regulation. Here we used modified λ plasmid, pTCλ1. advantage that it different numbers depending on concentration an inducer stimulates initiation replication. Results obtained with in recombination proficient deficient cells generally support but also suggest some modification model.