A computationally directed screen identifying interacting coiled coils from Saccharomyces cerevisiae

摘要: Computational methods can frequently identify protein-interaction motifs in otherwise uncharacterized open reading frames. However, the identification of candidate ligands for these (e.g., so that partnering be determined experimentally a directed manner) is often beyond scope current computational capabilities. One exception provided by coiled-coil interaction motif, which consists two or more α helices wrap around each other: sequences are generally other sequences, thereby greatly simplifying motif/ligand recognition problem. Here, we describe two-step approach to identifying protein–protein interactions mediated two-stranded coiled coils occur Saccharomyces cerevisiae. Coiled from yeast genome first predicted computationally, using multicoil program, and associations between then two-hybrid assay. We report 213 unique 162 putative sequences. evaluate resulting interactions, focusing on identified components spindle pole body (the centrosome).