Estrogen receptor β protects against in vivo injury in RPE cells

摘要: Epidemiological data suggest that estrogen deficiency in postmenopausal women may contribute to the severity of AMD. We discovered 17beta-estradiol (E2) was a crucial regulator extracellular matrix turnover (ECM) dysregulation both vivo and vitro. also found vitro presence receptor (ER)beta regulates MMP-2 activity. Therefore an attempt delineate role ER subtypes, female knockout (ERKO) mice were fed high-fat diet, eyes exposed seven 5-second doses nonphototoxic levels blue-green light over 2 weeks. Three months after cessation blue treatment, transmission electron microscopy performed assess deposits, Bruchs membrane changes, choriocapillaris endothelial morphology. changes trimolecular complex pro-MMP-2, MMP-14 TIMP-2 correlated with increased Bruch's thickening or sub-retinal deposit formation (basal laminar deposits) ERKObeta mice. In addition RPE isolated from had increase expression total collagen decrease Finally we ERK intermediate signaling molecule MMP pathway activated These which lack ERbeta are more susceptible injury associated environmental high fat diet.