Extraction of RNA from Ca-alginate-encapsulated yeast for transcriptional profiling.
作者： Arthur L. Kruckeberg , Saisubramanian Nagarajan , Kate McInnerney , Frank Rosenzweig
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摘要: We have developed a method for preparing high-quality total RNA from Ca-alginate-encapsulated Saccharomyces cerevisiae that is suitable microarray analysis. Encapsulated cells were harvested immobilized cell reactors and flash-frozen in liquid nitrogen. Following low-temperature mechanical disruption, freed Ca-alginate by reverse ionotropic gelation purified centrifugation, then was extracted using hot acid phenol. The yield quality of the consistently high; free contaminating alginate, analysis it performed as well isolated planktonic cells.
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