Use of selection pressures to enable microbial biosynthesis of polyhydroxyalkanoates from anaerobic degradation products

摘要: A method for inexpensive and efficient PHA biosynthesis includes operating a sequencing bioreactor in alternating phases of nutrient deprivation carbon feedstock to select robust PHA-producing microbes. Preferably, the is operated non-sterile manner with mixed cultures methanotrophs. The also preferably uses periodic biomass-wasting (PHA harvesting) at end feed phase, gradually lengthening time period phase create penalty rapid degradation incentive accumulation. Also, bacterial enrichment may be introduced periodically. PHA-accumulating bacteria are grown on common anaerobic products, specifically volatile fatty acids, such as acetate propionate, methane gas. has useful applications bioplastics other products.