Characterization of the cellulolytic bacteria communities along the gastrointestinal tract of Chinese Mongolian sheep by using PCR-DGGE and real-time PCR analysis.

摘要: A balanced gastrointestinal microbial ecosystem is crucial for the health and growth of animals. In tract (GIT) ruminants, cellulolytic bacteria aid in digestion absorption nutrients. Rumen contents feces ruminants are often used to assess communities; however, these sites do not guarantee represent diversity microbes found entire GIT. this study, we investigated microbiota along GIT five Chinese Mongolian sheep using PCR-denaturing gradient gel electrophoresis (DGGE) real-time PCR analysis. Results indicated that were more abundant stomach large intestine than small intestine. DGGE revealed predominance Firmicutes Bacteroidetes Meanwhile, Ruminococcus flavefaciens Clostridium cluster IV showed significant difference their abundance (P < 0.05). Fibrobacter succinogenes was most dominant species, followed by albus R. flavefaciens. The ileum harbored a larger number bacteria, particularly—Clostridium IV, reported previously. addition, comparisons between rumen rectum similar total Firmicutes, Bacteroidetes, F. succinogenes, Butyrivibrio fibrisolvens, XIVa, whereas higher rumen. This study composition quantification community sheep, first time bacterial sheep.