Mechanism and applications of new fluorescent compounds produced by femtosecond laser surgery in biological tissue (Conference Presentation)

摘要: The single or multi-photon microscopy based on fluorescent labelling and staining is a sensitive quantitative method that widely used in molecular biology medical research for variety of experimental, analytical, quality control applications. However, label-free highly desirable medicine when performing long term live imaging biological system obtaining instant tissue examination during surgery procedures. Recently, our group found femtosecond laser turned tissues protein samples into substances. newly formed compounds produced the can be excited via single- two-photon processes over broad wavelength ranges. We developed combined confocal spectroscopic microscope to characterize fluorescence from new compound systematically. structures treated were studied using Raman spectroscopy transmission electron microscopy. Our study revealed mechanisms emission form compound. Furthermore, we demonstrated applications evaluation microsurgery, stem cell responses muscle injury neuro-regeneration after spinal cord injury.