The Shewanella oneidensis MR-1 Fluxome under Various Oxygen Conditions

摘要: The central metabolic fluxes of Shewanella oneidensis MR-1 were examined under carbon-limited (aerobic) and oxygen-limited (micro-aerobic) chemostat conditions using 13C labeled lactate as the sole carbon source. labeling patterns key amino acids in biomass probed both GC-MS 13C-NMR. Based on genome annotation, a pathway model was constructed to quantify flux distributions. showed that tricarboxylic acid (TCA) cycle is major metabolism route conditions. Entner-Doudoroff pentose phosphate pathways mainly utilized for synthesis (flux below 5 percent uptake rate). anapleurotic reactions (pyruvate malate oxaloacetate phosphoenolpyruvate) glyoxylate shunt active. Under conditions, substantial amount oxidized via highly reversible serine pathway. Fluxes through TCA less whereas acetate production more oxygen limitation than limitation. Although distributions aerobic, micro-aerobic, shake-flask culture dramatically different, relative ratios did not vary significantly. Hence, S. appears be quite robust environmental changes. Our study also demonstrates merit coupling with NMR analysis reduce use substrates obtain accurate values.