Human mesenchymal stem cell viability, proliferation and differentiation potential in response to ceramic chemistry and surface roughness
作者： Patricia Mazón , David García-Bernal , Luis Meseguer-Olmo , Francesca Cragnolini , Piedad N. De Aza
DOI: 10.1016/J.CERAMINT.2015.01.110
关键词:
摘要: Abstract We investigated the effect of ceramic chemistry and surface roughness pure α-tricalcium phosphate, also αTCP doped with either 1.5 wt% or 3.0 wt% dicalcium silicate (C 2 S), on response adult human mesenchymal stem cells ( ah MSCs). A h MSCs were plated onto discs, prepared by a solid-state reaction. After being sintered, some samples polished up to 1 μm, while others kept as manufactured, which resulted in two grades. Viability, proliferation osteoinductive capacity determined following various incubation periods. The results showed non-cytotoxic after an indirect apoptosis test. Cell adhesion roughness-sensitive increased proportionally materials. These observations became more evident unpolished C S, induced osteoblastic differentiation result concentration S solid solution αTCP.
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sci-hub.se 参考文章(50)
N NISHIMURA, T KAWAI, Effect of microstructure of titanium surface on the behaviour of osteogenic cell line MC3T3-E1. Journal of Materials Science: Materials in Medicine. ,vol. 9, pp. 99- 102 ,(1998) , 10.1023/A:1008851115846
A. El-Ghannam, P. Ducheyne, I. M. Shapiro, Effect of serum proteins on osteoblast adhesion to surface-modified bioactive glass and hydroxyapatite. Journal of Orthopaedic Research. ,vol. 17, pp. 340- 345 ,(1999) , 10.1002/JOR.1100170307
José E. Maté‐Sánchez de Val, Jose L. Calvo‐Guirado, Rafael A. Delgado‐Ruiz, Mª P. Ramírez‐Fernández, Bruno Negri, Marcus Abboud, Isabel M. Martínez, Piedad N. de Aza, Physical properties, mechanical behavior, and electron microscopy study of a new α-TCP block graft with silicon in an animal model. Journal of Biomedical Materials Research Part A. ,vol. 100, pp. 3446- 3454 ,(2012) , 10.1002/JBM.A.34259
R. García Carrodeguas, A.H. De Aza, J. Jimenez, P.N. De Aza, P. Pena, A. López-Bravo, S. De Aza, Preparation and In Vitro Characterization of Wollastonite Doped Tricalcium Phosphate Bioceramics Key Engineering Materials. pp. 237- 240 ,(2007) , 10.4028/WWW.SCIENTIFIC.NET/KEM.361-363.237
Joel W. Reid, Loughlin Tuck, Michael Sayer, Karen Fargo, Jason A. Hendry, Synthesis and characterization of single-phase silicon-substituted α-tricalcium phosphate Biomaterials. ,vol. 27, pp. 2916- 2925 ,(2006) , 10.1016/J.BIOMATERIALS.2006.01.007
Haitong Zeng, Krishnan K. Chittur, William R. Lacefield, Analysis of bovine serum albumin adsorption on calcium phosphate and titanium surfaces. Biomaterials. ,vol. 20, pp. 377- 384 ,(1999) , 10.1016/S0142-9612(98)00184-7
Isabel M. Martínez, Pablo Velásquez, Luis Meseguer-Olmo, Piedad N. De Aza, Production and study of in vitro behaviour of monolithic α-tricalcium phosphate based ceramics in the system Ca3(PO4)2–Ca2SiO4 Ceramics International. ,vol. 37, pp. 2527- 2535 ,(2011) , 10.1016/J.CERAMINT.2011.03.062
Thomas J Webster, Richard W Siegel, Rena Bizios, Osteoblast adhesion on nanophase ceramics Biomaterials. ,vol. 20, pp. 1221- 1227 ,(1999) , 10.1016/S0142-9612(99)00020-4
Vladimir Murashov, Martin Harper, Eugene Demchuk, Impact of silanol surface density on the toxicity of silica aerosols measured by erythrocyte haemolysis. Journal of Occupational and Environmental Hygiene. ,vol. 3, pp. 718- 723 ,(2006) , 10.1080/15459620601015950
Ioannis D. Xynos, Alasdair J. Edgar, Lee D.K. Buttery, Larry L. Hench, Julia M. Polak, Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis. Biochemical and Biophysical Research Communications. ,vol. 276, pp. 461- 465 ,(2000) , 10.1006/BBRC.2000.3503