An ex vivo Approach to Study Hormonal Control of Spermatogenesis in the Teleost Oreochromis niloticus.

摘要: As the male reproductive organ, main task of testis is production fertile, haploid spermatozoa. This process, named spermatogenesis, starts with spermatogonial stem cells, which undergo a species-specific number mitotic divisions until starting meiosis and further morphological maturation. The pituitary gonadotropins, luteinizing hormone follicle stimulating hormone, are indispensable for vertebrate but we still far from fully understanding complex regulatory networks involved in this process. Therefore, developed an ex vivo cultivation system allows evaluating occurring changes histology gene expression after gonadotropin treatment. established circulatory organ culture explants Nile tilapia allowed examining effect extract (tilPE) human chorionic (hCG) on tissue. experimental flow-through setup described work provides possibility to study function gonads cellular transcriptional level at least seven days. After one week culture, slices kept their structure all stages spermatogenesis could be detected histologically. Without supplementation however, fibrotic structures appeared, whereas addition tilPE preserved spermatogenic cysts somatic interstitium completely. We show that has stimulatory spermatogonia proliferation our system. In presence or hCG, steroidogenesis related genes (cyp11b2 stAR2) were notably increased. Other testicular like piwi1, amh dmrt not expressed differentially absence gonadotropins. suitable studying promise future applications.