Differential binding of lectins to M cells and enterocytes in the rabbit cecum.

摘要: Abstract Background: The afferent limb of the intestinal immune system is represented by gut-associated lymphoid tissue, in which antigenic material, including complete bacteria, taken up from lumen specialized epithelial cells (M cells). Because adherence micro-organisms to epithelia can be mediated lectin-sugar bindings, glycoconjugates surfaces M and enterocytes were compared. Methods: A set 28 lectins corresponding sugars was used for light electron microscopy fixed unfixed sections. identified anti-vimentin antibodies. Results: cecal patches selectively bound specific fucose or N -acetylgalactosamine. labeled located apical membrane vesicles cytoplasm. Enterocytes galactose-specific lectins. In contrast, lectin-reactivity did not differ jejunal Peyer's patches. Conclusions: results suggest that there may selectivity uptake material but cells.