Development and testing of a combined species SNP array for the European seabass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata).

摘要: Abstract SNP arrays are powerful tools for high-resolution studies of the genetic basis complex traits, facilitating both selective breeding and population genomic research. The European seabass (Dicentrarchus labrax) gilthead seabream (Sparus aurata) two most important fish species Mediterranean aquaculture. While programmes increasingly underpin stocky supply this industry, selection is not yet widespread. Genomic has major potential to expedite gain, particularly traits practically impossible measure on candidates, such as disease resistance filletcharacteristics. aim our study was design a combined-species 60 K array seabream, test its performance farmed wild populations from numerous locations throughout range. To achieve this, high coverage Illumina whole genome sequencing pooled samples performed 24 27 seabream. This resulted in database ~20 million SNPs per species, which were then filtered identify high-quality variants create final set development ‘MedFish’ array. tested by genotyping subset discovery populations, highlighting conversion rate functioning polymorphic assays (92% seabass: 89% seabream) repeatability (99.4–99.7%). platform interrogates ~30 K markers each includes features previously shown be associated with enriched predicted have functional effects proteins. demonstrated effective at detecting structure across wide range diverse geographical origins, examine extent haplotype sharing among populations. In conclusion, new MedFish enables efficient accurate high-throughput genome-wide distributed will facilitate stock management, genomics approaches, acceleration through selection.