The study of neuroprotective effect of ferulic acid based on cell metabolomics.

摘要: Abstract Ferulic acid (FA), a naturally derived phenolic compound, has antioxidant and antidepressant-like effects. It is still challenge to study its mechanism due the complexity of pathophysiology depression. In this study, ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used perform metabolomics studies based on biochemical changes in differentiated rat pheochromocytoma (PC12) cells treated corticosterone-induced neurological damage after FA treatment. A total 31 metabolites were identified as potential biomarkers for PC12 cells injury. Among them, 24 regulated Pathway analysis revealed that these mainly involved amino metabolism, energy metabolism glycerophospholipid metabolism. addition, results metabolomics, three cell signaling pathways related glutamate discovered. To further interactions between major targets pathways, molecular docking method employed. The showed had strongest binding power protein kinase B (AKT). Furthermore, result mRNA analyzed by quantitative real time RT-PCR indicated AKT (PKA) pathway up treatment compared model group. This shows strategies associated biology helpful tool elucidate neuroprotective FA.