Excess salt exacerbates blood-brain barrier disruption via a p38/MAPK/SGK1-dependent pathway in permanent cerebral ischemia

摘要: High salt diet (HSD) is one of the most important risk factors that contribute to many vascular diseases including ischemic stroke. One proposed mechanism underlying disruption blood-brain barrier (BBB) mediated by HSD indirectly through enhancing blood pressure. The direct role on BBB integrity unclear. Our purpose determine whether and how might be involved in breakdown during ischemia. To test that, we induced model cerebral ischemia permanent middle artery ligation (pMCAL) either normal or fed mice. We observed significantly enhanced brain damage which was associated with disruption, increased leukocytes infiltration loss tight junction (TJ) proteins expression without apparently altering vitro experiment also revealed sodium chloride (NaCl) treatment down-regulated TJ protein endothelial cells substantially permeability starvation. Inhibition p38/MAPK/SGK1 pathway eliminated effect NaCl vitro. In addition, noticed a positive correlation between urinary levels lesion size stroke patients. Together, our study demonstrates hypertension-independent provides rationale for post attack management.