Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus

摘要: Lysine biosynthesis of Thermus thermophilus proceeds in a similar way to arginine biosynthesis, and some lysine biosynthetic enzymes from T. so far investigated have the potential function biosynthesis. These observations suggest that might regulate expression genes for To test this hypothesis, argR gene encoding regulator was cloned its analysed. The addition culture medium inhibited growth an arginase knockout mutant thermophilus, which presumably accumulates inside cells. Arginine-dependent inhibition not alleviated by ornithine, is intermediate serves as peptidoglycan component cell wall thermophilus. However, cancelled either simultaneous ornithine or gene, suggesting involvement regulation Electrophoretic mobility shift assay DNase I footprinting revealed ArgR protein specifically binds promoter region major cluster. Furthermore, α-galactosidase reporter indicated repressed argR-dependent manner. results indicate regulated